Reporter

Part:BBa_K4799606:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pNtCCX2_GUS_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1910
    Illegal SpeI site found at 2207
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1910
    Illegal NheI site found at 4016
    Illegal SpeI site found at 2207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1910
    Illegal BglII site found at 1998
    Illegal BamHI site found at 1981
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1910
    Illegal SpeI site found at 2207
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1910
    Illegal SpeI site found at 2207
    Illegal NgoMIV site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2231
    Illegal BsaI.rc site found at 2846
    Illegal SapI.rc site found at 3017


Design Notes

Test if the promoter will induce GUS express after cadmium treatment.



Source

plasmid

References